Staff at The Parchment Makers, Havant are dressed in blue clothes.
They are also holding a raffle and are selling tickets until Monday.
Pub manager Mark Slingsby said: ‘We’d love to raise as much as possible.
‘It really depends on how many tickets we sell.’
Employees hope to raise at least £200 for the Tom Prince Cancer Trust, and were the very first place to sign up for Blue Day this year.
All proceeds from Blue Day go to the Tom Prince Cancer Trust.