The numbers were so high that police decided to close the road to cars.
The event was held to celebrate Albert Road, Southsea, and encourage people to use bikes around Portsmouth.
It was also part of Blue Week – the run-up to Blue2Day – and collectors were out with buckets to rake in cash for the charity.
The money hasn't yet been counted up, but it's thought to amount to hundreds of pounds.
Funds were also raised for the British Heart Foundation.
Portsmouth Joggers visited the event with the Blue Torch running along Albert Road in their quest to run 83 miles around Portsmouth – the distance between Fratton Park and Wembley.
The torch is being carried to Blue events around Portsmouth in the run-up to Saturday's FA Cup final.
Up to 8,000 people attended the day – an estimated 2,000 of whom were riding push bikes.
The street saw everything from rickshaws to a penny farthing, ridden by event organiser Ian Parmiter, who owns an antiques shop in the road.
Ian said: 'It was a great day. We held it to celebrate the road, because it's got some great shops, restaurants and bars and is a really great place.
'It's good that the day was also part of Blue Week, it's a really good cause – I dropped £100 in the bucket myself. Hopefully a lot of money was raised.'
Joy Page, 47, of Bramshott Road, Southsea, spent the afternoon in Albert Road with her two-year-old Jack Russell, Kipper, in the basket of her bike.
She said: 'Kipper really loved it and was getting lots of attention.'
There were also bands and buskers at the event.