Pompey Reserves will face Sheffield United in the last 16 of the Premier League Cup.
The Blues’ second team will take on the Blades on home soil between March 17-20.
Full details of the tie are still to be confirmed.
Pompey progressed to the knockout stages of the competition after beating Everton 2-1 at Westleigh Park last month.
Goals from Ben Close and Nicke Kabamba earned them victory.