Emma Boothroyde, 38, and her daughter Elouise Paterson, nine, had the snip at the family business, the Newtown House Hotel on Hayling Island.
Their hair was cut by Rachael Simms, 22, who is the head receptionist and a qualified hairdresser.
The nine inches from Emma and eight from Elouise will be donated to the Little Princess Trust, which provides real-hair wigs, free of charge, to boys and girls who have lost their own hair through cancer treatment and other illnesses.
Emma said: ‘My daughter had the initial idea.
‘She wanted to donate it to children to make the wigs.’
Emma said she was ‘shocked but happy’ with her new look.