Gosport Borough crashed out of the FA Trophy following a 3-1 defeat at the hands of AFC Totton.
It was Alex Pike’s side’s second cup exit of the week, after they were eliminated from the FA Cup by Slough Town on Tuesday night.
Home-side Totton took the lead after nine minutes and never looked back.
Richard Gillespie ran through to meet a sublime pass and beat keeper Nathan Ashmore at his near post with a fine shot.
Gosport suffered another early blow minutes later when Luke King was stretchered off after catching his studs in the turf and twisting his ankle.
He was then taken to Southampton General Hospital for further tests.
Totton then doubled their lead when a long-range free-kick from Ryan Hill came down off the bar and bounced out.
But the assistant on the far side had seen it cross the line and flagged, leading to the ref rewarding the goal.
The second half saw Gosport fight back, with Tom Dunford rifling home a shot through a crowded penalty area.
But with the hard work done, Gosport then conceded a third which proved to be the winner.
The visitors’ defence failed to deal with a cross from the right and Jamie Whisken was given a free header to place the ball past Ashmore.
Alex Pike made two substitutions, bringing on Sammy Igoe and Adam Wilde for Dan Wooden and Dan Woodword.
And Wilde had an immediate impact, latching on to a rebounded shot to fire home from just inside the six-yard box.
But despite a full-scale assault on the Totton goal, Gosport were unable to find a way through and crashed out of the FA Trophy.
Gosport: Asmore, Woodward, Poatte, Cooke, Molyneaux, Dunford, Brown, Smith, Wooden, Bennett, King. Sunbsd: Wilde, Gaisie, Igoe, Martin, Stow.