Horndean had their FA Cup hopes dashed by a contentious goal in a 1-0 defeat by Newport IOW at Five Heads Park.
Deans boss Dave Carter felt the officials missed a clear offence before the visitors scored.
He said: ‘Their two players were at least four yards offside and one of them clearly handled the ball.
‘All our players stopped, expecting a whistle, but it didn’t come.
‘It is hugely disappointing to go out because of one mistake by the officials.
‘Frustratingly, we also felt we should have had a penalty late on.’
The decisive goal was scored in the 20th minute when Tom McInnes netted.
Moneyfields consolidated their position at the top of the Wessex League premier division with a 3-0 win at Andover Town.
Goalkeeper Cameron Scott saved an early penalty.
Moneys then stormed into a three-goal lead.
Steve Hutchings grabbed a brace, with Jake Morgan completing the scoring – all inside 30 minutes.
‘We are playing well at the moment and there is a buzz about the squad,’ said Moneys boss Miles Rutherford.
Petersfield Town recorded a 2-1 victory at Lymington Town.
Goals from Warren Hunt and substitute Andy Todd proved decisive – but an injury to striker Robbie Tambling could prove a major blow for the Rams.
Petersfield boss Ian Saunders reckoned his team were not at their best, despite the win.
He said: ‘It was probably our worst performance of the season.
‘Lymington were physical and robust but we stood up to them and came away with the points.
‘It looks like we have lost Robbie Tambling, with a damaged shoulder for a while though, after he was sent crashing into the hoardings.’