Ben Davies has been ruled out of facing former club Pompey.
The right-back is not yet ready to return from the calf problem which has sidelined him since October.
Davies has missed Grimsby’s last nine matches and, despite this week returning to training, the fixture has come too soon.
Also missing is midfielder Dominic Vose, who collected a thigh injury in last weekend’s 3-2 defeat at Crawley.
Long-term absentee Sean McAllister is once again absent from the Mariners’ plans.