Goals from Charley Wilson, Jess Frampton and Gemma Simmonds earned Pompey Ladies a 3-2 win at Keynsham in the FA Premier League Cup.
Perry Northeast made five changes to his starting line-up from the opening league win at Cardiff.
He has increased strength in depth this season and the rotation paid off as the team progressed through the determining round tie.
Simmonds hit the winner in the 74th minute of the match.
Earlier, Frampton opened the scoring and Wilson made it 2-0.
Keynsham then battled back to level.
But it was Pompey’s day.