Their previous encounter resulted in a slap to the face and the brandishing of a red card.
Now Nathan Thompson and Alex Mowatt are limbering up to lock horns in battle once more.
But Kenny Jackett is adamant there will be no lingering animosity from Pompey’s end ahead of Saturday’s Oakwell clash.
The League One leaders travel to Barnsley seeking the resumption of winning ways after eight-successive victories were halted by Charlton in the week.
The fixture also matches Thompson up against Mowatt for the first time since their March head-to-head at Fratton Park.
Then on loan at Oxford United, Mowatt planted a 62nd minute penalty against the post, prompting Thompson to goad him from close range.
The midfielder responded by striking the Blues right-back in the face – sparking his dismissal as the hosts went on to triumph 3-0.
Mowatt has since returned to Barnsley, establishing himself as an integral part of a side which presently sits in sixth position.
Now they will meet once more – but Jackett isn’t anticipating any further trouble.
Pompey’s boss said: ‘I don’t think there’ll be anything.
‘Nathan won’t, he’s professional, he’s competitive, but I don’t think either player will be thinking about that.
‘From our side, no there won’t be. From Nathan or myself, there won’t be anything.
‘That sending off changed the game against Oxford, it was nip and tuck at the time. From our side, there won’t be carried over.
‘I didn't necessarily think Nathan playing at right-back and Mowatt in the centre of midfield in a 4-4-2 will come up against each other.
‘But from Nathan or for myself, there will be no carry over at all.’
Mowatt, who appeared 34 times for Oxford during a season-long loan, has been a league ever-present for the Tykes this term.
He has netted twice in 25 outings and is regarded as their most impressive performer so far this campaign.
And Jackett recognises the ex-Leeds man’s talents.
He added: ‘Barnsley have had a good season so far, they have a good team.
‘Everybody knows about Mowatt’s ability, he's a good player.
‘The age he is, they have taken him in and he is a good midfield player for them.’