It represents the 29-year-old’s first start in 10 matches - and he lines-up alongside striking rival George Hirst.
It was Marquis’ stoppage-time headed winner which earned triumph over the Grecians at the competition’s semi-final stage in February 2020.
And he has been recalled for Danny Cowley’s side in a strong starting XI for the Blues.
Ronan Curtis' Portsmouth future spelt out by trusted source well in the know - with advice on where to play Irishman added, too
Former Portsmouth keeper Alex Bass suffers tough night on and off the pitch as he makes Sunderland debut following Fratton Park exit
Portsmouth's Miniesta bidding to live up to big reputation - and those comparisons with ex-Barcelona and Spain star
'We wanted to show him a bit of love' - Portsmouth-based Spurs fans' terrific gesture to ensure Dane Scarlett feels at home on the south-coast
The most expensive estimated League One combined XI - including two Portsmouth, four Barnsley, one Oxford United and two Derby aces
However, there is no sign of Ellis Harrison, who is in talks over a permanent move away from Fratton Park.
While Gassan Ahadme, who will return to Norwich this month with the cancellation of his loan, is not included in the match-day 18.
Elsewhere, Gavin Bazunu is rested, so Alex Bass comes in, while Louis Thompson is fit enough to be named on the bench.
However, Clark Robertson and Ryan Tunnicliffe are still not ready to return to match-day duty following injury.
Meanwhile, Pompey’s bench includes Academy pair Toby Steward and Harry Jewitt-White.
Pompey: Bass, Freeman, Ogilvie, Raggett, Romeo, Brown, Morrell, Azeez, Harness, Marquis, Hirst,
Subs: Steward, Curtis, Hackett, Thompson, Jacobs, Downing, Jewitt-White.
A message from the Editor, Mark Waldron
You can support our local team of expert Pompey writers by subscribing here for all the latest news from Fratton Park for 9p a day, thanks to our 30%-off transfer window deal.