Gosport & Fareham begin their London one south travels, after two home games, with a visit to Twickenham (3pm).
Coach Neil McRoberts has been pleased with the way his team have coped with the step up after last season’s promotion.
They followed up a narrow opening-day bonus-point defeat with a blockbuster 27-18 derby win over Havant.
‘The squad is very upbeat after playing well to beat Havant,’ said McRoberts.
‘Now it depends on how well we travel in what is not an easy first away game.
‘We need to make sure we carry on where we left off in the Havant game.
‘Last time we played Twickenham it proved a forward battle and we are expecting the same again.
‘We have to make sure we front up as we did against Havant.’
The visitors have to do without three of last week’s pack as Chris Thompson, Mark Jones and Tom Blackburn are in New Zealand with the Royal Navy.
Sam Foster returns to the centre for the first time this season after holiday.
Fareham Heathens visit Team Solent looking to build on their 20-13 season-opening win over Bognor in Hampshire one.
Ambitious Locks Heath Pumas open up their Hampshire two campaign at Alresford (3pm).
The Pumas narrowly missed out on promotion last season and coach Jim Pearce believes his team can go one better this time around.
‘We will be disappointed if we are not up there challenging,’ said Pearce.
A number of new players have been recruited during the summer with a few expected to make their debuts.
Second-row Matt Thurston, back-row forward Tom Palmer, fly-half Ben Chambers and winger Stuart Forbes could all make their first appearance.
Back-row forward Miles Carter also returns after missing all but 20 minutes of last season with a medial ligament injury.
Ex-Gosport & Fareham centre Liam Moggeridge captains the side and Pearce may also be able to call upon the experience of former Havant forward John Barfoot.
‘The ability of the guys coming in has raised the level,’ said Pearce.
‘There players are going to have to fight for their places.’
Southsea Nomads start with a long trip to Chineham.