Gosport & Fareham slumped to a heavy 46-20 defeat at Twickenham in London two south west.
‘After finding ourselves 14-7 down in the first half we got in the ascendancy but made the fundamental mistake of missing too many tackles,’ said director of rugby Jamie Daly.
‘We had bounced back and were dominant in the forwards.
‘Unfortunately we were unable to make our good phases of play count.
‘Then two or three lapses in the space of as many minutes saw us concede three tries.
‘We had the wind behind us in the second half and believed we could get back into it.
‘But the second period was too scrappy and whether the players were trying too hard or just missed tackles I am not really sure.
‘We can take a lot of positives because we scored three tries but there are also quite a number of negatives to work on.’
In the first half, Wayne Dugan and Mark Pollard crossed for Gosport tries, both converted by Alex Duncombe.
After the break the visitors only added two more Duncombe penalties.
In London three south west, Petersfield were crushed 65-9 by second-placed Camberley.
United Services stormed to an impressive 38-29 win over third-placed Millbrook in their Hampshire one contest at Burnaby Road.
Fareham Heathens crashed to a 46-5 defeat at Farnborough.
Meanwhile, Portsmouth under-15s overcame Petersfield under-15s 19-12.
Hugo James-Warner and Felix Tuff scored tries for the visitors and Thomas Gillett adding one conversion.